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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Microvesicles from bone marrow-derived mesenchymal stem cells promote Helicobacter pylori-associated gastric cancer progression by transferring thrombospondin-2

Fig. 4

MVs derived from BMSCs exposed to H. pylori express higher protein levels of THBS2. A BMSCs were transfected with scrambled shRNA (negative control) or THBS2 shRNA. Transfection was confirmed by the presence of red fluorescent protein. B Western blotting confirmation of successful THBS2 depletion following THBS2-specific shRNA transfection (sh-THBS2) compared with NC transfection (sh-NC). C Transmission electron microscopy images of sh-NC-MVs, Hp + sh-NC-MVs, sh-THBS2-MVs and Hp + sh-THBS2-MVs; scale bar = 50 nm. D Representative immunoblot bands of THBS2 in sh-NC-MVs, Hp + sh-NC-MVs, sh-THBS2-MVs and Hp + sh-THBS2-MVs. E Representative immunofluorescence confocal laser images of BMSC-MVs (red) and MGC-803 cells (green) and the nuclei (blue), scale bar = 50 μm. F Representative immunofluorescence confocal laser images of BMSC-MVs (red) and THBS2 (green), the nuclei (blue), Scale bar = 50 μm. BMSCs: bone marrow-derived mesenchymal stem cells; MVs: microvesicles; Hp: Helicobacter pylori; GC: gastric cancer; NC: negative control. **P < .01, ***P < .001, ****P < .0001

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Cell Communication and Signaling

ISSN: 1478-811X