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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: A novel interface between the N-terminal and coiled-coil domain of STAT1 functions in an auto-inhibitory manner

Fig. 5

The STAT1-E169A mutation increases transcriptional activity of target genes. A U3A cells coexpressing fusion proteins of WT or mutant STAT1 with a luciferase reporter gene coupled to a 3xLy6E, pIC-339 or pIC-1352 promoter were stimulated for 6 h with 50 ng/ml IFNγ. The data are normalized to β-galactosidase coexpression. B mRNA levels of endogenous STAT1 target genes in IFNγ-stimulated U3A cells expressing WT or mutant STAT1, obtained by qPCR. Asterisks indicate significant differences between the WT protein and the respective mutant

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Cell Communication and Signaling

ISSN: 1478-811X