Fig. 4

Nuclear accumulation of the STAT1-E169A mutant is accelerated after IFNγ stimulation. HeLa cells expressing fusion proteins of STAT1-WT (A) or the H158A (B) and E169A (C) mutants were either left unstimulated or stimulated with 50 ng/ml IFNγ for the indicated times (scale bar corresponds to 50 µm). D Quantification of the results from Fig. 4A–C. STAT1-GFP fluorescence intensity data from n = 20 cells are plotted as the ratio of nuclear-to-total STAT1. Asterisks indicate significant differences between the WT protein and the respective mutant