Fig. 3From: A novel interface between the N-terminal and coiled-coil domain of STAT1 functions in an auto-inhibitory mannerSTAT1-E169A retains high levels of tyrosine phosphorylation and nuclear localization. U3A cells expressing fusion proteins of WT (A) or mutant (B, C) STAT1 were either not stimulated or stimulated with 50 ng/ml IFNγ for 45 min before incubation with 1 µM staurosporine for the indicated times (scale bar corresponds to 50 µm). D Quantification of the results from Fig. 3A–C. Total fluorescence intensity of tyrosine-phosphorylated STAT1 was measured from n = 20 cells in each experiment. Asterisks indicate significant differences in the tyrosine-phosphorylation level between the WT protein and the E169A mutantBack to article page