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Fig. 9 | Cell Communication and Signaling

Fig. 9

From: TGF-β1 upregulates secreted protein acidic and rich in cysteine expression in human granulosa-lutein cells: a potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome

Fig. 9

SPARC knockdown downregulates SMAD4 expression. A-C KGN cells were transfected with 50 nM control siRNA (si-Ctrl) or SPARC siRNA (si-SPARC) for 48 h. The mRNA levels of SPARC and TGFB1 (A), ALK5 and TβRII (B), SMAD2, SMAD3, and SMAD4 (C) were examined by RT-qPCR. D KGN cells were transfected with 50 nM control siRNA (si-Ctrl) or SPARC siRNA (si-SPARC) for 48 h. The protein levels of SMAD4 were examined by western blot. The results are expressed as the mean ± SEM of at least three independent experiments. Values that are statistically different from one another (p < 0.05) are indicated by different letters

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Cell Communication and Signaling

ISSN: 1478-811X