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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: TGF-β1 upregulates secreted protein acidic and rich in cysteine expression in human granulosa-lutein cells: a potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome

Fig. 8

SPARC is required for TGF-β1-stimulated VEGF and aromatase expression in both primary hGL cells derived from control and OHSS patients. A and B Primary hGL cells derived from non-OHSS (A) and OHSS (B) were transfected with 50 nM control siRNA (si-Ctrl) or SPARC siRNA (si-SPARC) for 48 h, and then treated with 5 ng/mL TGF-β1 (T) for 3 h. The VEGF protein levels were examined by western blot. C and D Primary hGL cells derived from non-OHSS (C) and OHSS (D) were transfected with 50 nM control siRNA (si-Ctrl) or SPARC siRNA (si-SPARC) for 48 h, and then treated with 5 ng/mL TGF-β1 (T) for 24 h. The aromatase protein levels were examined by western blot. The results are expressed as the mean ± SEM of at least three independent experiments. Values that are statistically different from one another (p < 0.05) are indicated by different letters

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Cell Communication and Signaling

ISSN: 1478-811X