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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: TGF-β1 upregulates secreted protein acidic and rich in cysteine expression in human granulosa-lutein cells: a potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome

Fig. 1

TGF-β1 stimulates SPARC expression in KGN and primary hGL cells. A KGN cells were treated with 10 ng/mL TGF-β1 for different periods, and the mRNA levels of SPARC were examined by RT-qPCR. The level of SPARC mRNA at each time point was normalized to the GAPDH mRNA level at the same time point. B KGN cells were treated with 10 ng/mL TGF-β1 (T) for 6, 12, and 24 h, and the protein levels of SPARC were examined by western blot. C and D KGN cells were treated with 5 or 10 ng/mL TGF-β1 for 6 h. The SPARC mRNA levels (C) and protein levels (D) were examined by RT-qPCR and western blot, respectively. E Primary hGL cells were treated with 5 ng/mL TGF-β1 (T) for 6, 12, and 24 h, and the protein levels of SPARC were examined by western blot. The results are expressed as the mean ± SEM of at least three independent experiments. Values that are statistically different from one another (p < 0.05) are indicated by different letters

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Cell Communication and Signaling

ISSN: 1478-811X