Fig. 7

APTO253 treatment induces expression of KLF4 and MICA but represses MYC in AML cell lines. A HL60, NB4, and MM6 cell lines were incubated with APTO253 at concentrations indicated for 18 h followed by RT-qPCR to measure KLF4, MICA, and MYC expression. Data are shown as the mean (± SEM) of three independent experiments. B Expression level of surface MICA on untreated, LBH589- or APTO253-treated HL60 cells measured by flow cytometry. A histogram of one experiment and the mean (± SEM) of three biological replicates is depicted. Significance was calculated using an unpaired t-test (A,B). C HL60 cells, either treated with APTO253 or solvent were incubated with NK cells in a ratio of 1:10 as described in C) and the supernatant of mono- and co-cultures was used to measure released IFNγ and TNFα by ELISA. n = three technical replicates. D No treatment (control vehicle) and APTO253-treated HL60 cells were used as target cells in an NK cell-dependent killing assay using NK cells isolated from healthy donors (one out of three representative experiments). The ratio of target cells to effector NK cells is indicated (E:T). The basal cell death of HL60 ± APTO253 without any NK cells was measured in parallel to the co-cultured HL60. These values ranged from ~ 2–10% and were then subtracted from the co-culture values. E Graphical summary