Fig. 5

LBH589-mediated MICA upregulation is diminished in KLF4-depleted cells. A Cells were treated with 5 µM or 10 µM KLF4 inhibitor kenpaullone (ken) for 4 h prior to LBH589 treatment and subjected to RT-qPCR to measure MICA mRNA. See supplemental Western Blot Figure S3 for KLF4 protein expression. B HEK293 cells were treated with siRNA (control and KLF4) for 24 h followed by 100 nM LBH589 4 h treatment. The expression of the KLF4 and MICA genes was measured by RT-qPCR analysis. Data represent the mean (± SEM) of two biological experiments each measured in duplicates. Significance was calculated using a Mann–Whitney test. C HL60, NB4, and MM6 AML cell lines were treated with 100 nM LBH589 for 4 h and RT-qPCR was performed to detect mRNA expression levels of MICA and KLF4, respectively. The mean (± SEM) of three or four independent experiments is indicated and significance was calculated using an unpaired student’s t-test