Fig. 3

EnChiP-MS targeting the MICA locus. A Candidate proteins, putatively involved in MICA regulation (full list see Supplement Table S1). Factors with red edges are already identified regulators and transcription factors with binding motifs in the MICA promoter or/and factors regulated by CBP/p300. B Heat map of label-free quantitation of MS data of three biological replicates for the MICA (1,2,3) and the GFP control immunoprecipitations (4,5,6). Note the divergence of samples 1 and 6, reflecting experimental variation and/or the highly dynamic nature of chromatin. C Enrichment analysis to catalog proteins according to their cellular compartments using a functional enrichment analysis tool (http://www.funrich.org). About 50% of the proteins annotated to cytosol were also detectable in the nucleus and of these about 40% were annotated to the nucleolus. D Protein–protein interaction network for candidate transcription factors constructed using the STRING database (https://string-db.org). Associations are specific and meaningful but do not necessarily imply physical interaction. Interactions are indicated as experimentally determined (pink); from curated databases (blue); predicted from gene neighborhood (green), gene co-occurrence (dark blue), or protein homology (purple)