Fig. 1

LBH589 regulates the transcription of MICA from the standard promoter but not from the upstream promoter. A Section from the UCSC Genome Browser (Human, GRCh37/hg19) to depict the MICA upstream and standard promoter regions (red lines) and the corresponding exon 1 (blue boxes). The standard promoter loci, which are targeted by the guide RNAs are provided in the methods section. B RT-qPCR analysis to measure the expression level of the upstream transcript and the standard transcript in HEK293 cells treated with 100 nM LBH589 for 4 h. Data represent the mean ± SEM of three independent experiments. Significances were calculated using an unpaired student's t-test