Fig. 6

LOX-siRNA rescued hyperactivation of osteoclast function in female adult offspring rats induced by PDE. A Representative images of TRAP and F-actin staining of osteoclasts in the CON and PDE group with NC-siRNA or LOX-siRNA. B Quantification analyses of TRAP⁺ osteoclastic cells number per well. C: Quantification analyses of F-actin belt size per osteoclast. D-I NFATc1, c-Fos, Acp5, CtsK, Oscar, and DC-stamp mRNA expression in the CON and PDE group osteoclasts with NC-siRNA or LOX-siRNA. J NFATc1, c-Fos, and CtsK protein expression in osteoclasts in the CON and PDE group with NC-siRNA or LOX-siRNA. K-M Quantification analyses of NFATc1, c-Fos, and CtsK protein expression. Mean ± S.E.M., n = 3 per group for TRAP staining, F-actin staining and western blotting, n = 6 per group for mRNA expression. ^*P < 0.05, ^**P < 0.01 vs. CON + NC-siRNA. ^#P < 0.05, ^##P < 0.01 vs. PDE + NC-siRNA. PDE: prenatal dexamethasone exposure; TRAP: tartrate-resistant acid phosphatase; CON: control; NC-siRNA: negative control-small interfering RNA; NFATc1: nuclear factor of active T cells 1; c-Fos: protooncogene c-Fos; Acp5: acid phosphatase 5; CtsK: cathepsin K; Oscar: osteoclast-associated receptor; DC-stamp: dendritic cell-specific transmembrane protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase