Fig. 2

PDE induced inhibition of osteoclast function in fetal rats, while osteoclast function was not significantly altered in juvenile offspring rats. A, R Representative images of TRAP staining in decalcified bone sections of fetal rats and juvenile offspring rats. B, S Quantification analyses of N.Oc/B.Pm. C, T Quantification analyses of Oc.S/BS. D, U NFATc1, c-Fos, Acp5, CtsK, Oscar, and DC-stamp mRNA expression in bone tissue of fetal rats and juvenile offspring rats. E Representative images of TRAP and F-actin staining in osteoclasts treated with different concentrations of DEX. F Quantification analyses of TRAP⁺ osteoclastic cells number per well. G Quantification analyses of F-actin belt size per osteoclast. H-M NFATc1, c-Fos, Acp5, CtsK, Oscar, and DC-stamp mRNA expression in osteoclasts treated with different concentrations of DEX. N NFATc1, c-Fos, and CtsK protein expression in osteoclasts treated with different concentrations of DEX. O-Q Quantification analyses of NFATc1, c-Fos, and CtsK protein expression. Mean ± S.E.M., n = 3 per group for TRAP staining, F-actin staining, and western blotting, n = 8 per group for mRNA expression in vivo, n = 6 per group for mRNA expression in vitro. ^*P < 0.05, ^**P < 0.01 vs. Control in vivo or 0 nM DEX in vitro. PDE: prenatal dexamethasone exposure; TRAP: tartrate-resistant acid phosphatase; N.Oc/B.Pm: osteoclast number per bone perimeter; Oc.S/BS: osteoclast surface per bone surface; NFATc1: nuclear factor of active T cells 1; c-Fos: protooncogene c-Fos; Acp5: acid phosphatase 5; CtsK: cathepsin K; Oscar: osteoclast-associated receptor; DC-stamp: dendritic cell-specific transmembrane protein; DEX: dexamethasone; SDHA: succinate dehydrogenase; TBP: TATA box binding protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta