Fig. 1

IL-33-deficient mice have massive DNA accumulation and type 1 IFN production during APAP-induced injury. A H&E staining was used to observe hepatic necrosis at different times after APAP (250 mg/kg) administration (n = 6), scale bar = 100 µm. B Serum ALT/AST was detected in WT and IL-33.^−/− mice at 6, 12, and 24 h after APAP (250 mg/kg) administration injection (n = 6). C-E The mRNA levels of cGAS, STING and IFN-β1 were measured by qRT-PCR after 6 h, 12 h and 24 h APAP (250 mg/kg) treatment (n = 6). F Confocal microscopy showing DNA accumulation in the liver following APAP challenge (Sytox green staining; n = 3/group), scale bar = 100 µm. *P < 0.05, **P < 0.01, ***P < 0.001