Fig. 7
From: Phase separation propensity of the intrinsically disordered AB region of human RXRβ
Effects of TFE on the proteolysis of AB_hRXRB by proteinase K, trypsin and endoproteinase Glu-C (V8). Purified AB_hRXRB alone or in the presence of 30% TFE was digested with proteinase K (Lanes 4–5; 11–12 and 17–18), trypsin (Lanes 6–7; 13–14 and 19–20), or endoproteinase Glu-C (V8) (Lanes 8–9; 15–16 and 21–22). Proteolysis was conducted at 15 °C for an hour. The control samples were the intact AB_hRXRB without TFE (Lane 1) and that in the presence of 30% TFE (Lane 2). The molecular weight marker is shown on the left in kDa (Lanes 3 and 10). Aliquots were taken at 5 min (Lanes 4–9), 15 min (Lanes 11–16) and after 60 min (Lanes 17–22) after the addition of enzymes. The scheme in the lower panel depicts the putative protease cleavage sites of AB_hRXRB