Fig. 4
From: Phase separation propensity of the intrinsically disordered AB region of human RXRβ
Hydrodynamic properties of AB_hRXRB. A Sedimentation velocity analysis (SV-AUC). Superposition of the sedimentation coefficient c(s) distributions for three different concentrations of AB_hRXRB: 0.25 mg/ml (magenta line), 0.5 mg/ml (gray line) and 1.0 mg/ml (black line). B The plot presents the logarithmic values of the hydrodynamic radii (Rs) versus the logarithmic values of the molecular mass (M) for proteins in the native state (black squares), molten globule state (white triangle), premolten globule state (white squares), unfolded state (black triangle), coil-like proteins (white circle) and PMG-like proteins (gray squares). The data for globular proteins were taken from [60], and the data for IDPs are from [58]. The green circle corresponds to the Rs of AB_hRXRB calculated from the equation log (Rsnative) = (0.373 ± 0.014) × log (M)—0.274 ± 0.064, assuming it is a globular protein (AB_hRXRBtheor). The magenta diamond corresponds to the Rs determined for AB_hRXRB after AUC analysis (AB_hRXRBexp)