Fig. 11
From: Phase separation propensity of the intrinsically disordered AB region of human RXRβ
Factors influencing the formation of AB_hRXRB liquid condensates. A The phase behavior of AB_hRXRB in the presence of crowding agents. Representative images obtained by DIC of 260 μM AB_hRXRB in the presence of 10% (w/v) PEG 8000, 10% (w/v) PEG 6000 and 10% (w/v) PEG 3000. The addition of 10% (w/v) 1,6-hexanediol to the AB_hRXRB sample with 10% (w/v) PEG 8000 allowed for the droplets to dissolve. B The phase behavior of AB_hRXRB in the presence of 10% trehalose and 10% Ficoll 70. Representative images obtained by DIC of 165 μM AB_hRXRB. C AB_hRXRB droplet formation in the presence of TMAO. Incorporation of Atto 488-labeled AB_hRXRB (70 μM) into the droplets in the presence of 1.5 M TMAO. The addition of 20% (w/v) 1,6-hexanediol to the AB_hRXRB sample with 1.5 M TMAO dissolved the droplets. D The phase behavior of AB_hRXRB in the presence of kosmotropic salt. LLPS driven by 200 μM AB_hRXRB in the presence of 0.5 M (NH4)2SO4. The wetting properties of AB_hRXRB liquid droplets on the surface of a glass slide over time are also apparent. Scale bars: 10 μm