Fig. 6

Snail mediates the GDF-8-stimulated cell invasion. A and B, HTR-8/SVneo (A) and primary EVT (B) cells were pretreated with vehicle control (DMSO) or 10 µM SB431542 for 1 h, and then treated with 30 ng/mL GDF-8. C HTR-8/SVneo were transfected with 50 nM control siRNA (si-Ctrl) or Snail siRNA (si-Snail) for 48 h, and then treated with 30 ng/mL GDF-8. The cell invasiveness was measured by the Matrigel transwell invasion assay. For the invasion assay, the top panel shows representative photos of the invaded cells. The bottom panels show summarized quantitative results. D Representative images of immunohistochemical staining for MMP2 in the control and PE placentas. The scale bar represents 100 μm. The results are expressed as the mean ± SEM of at least three independent experiments. Multiple comparisons were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. Values that are statistically different from one another (p < 0.05) are indicated by different letters. The values with any common letter are not significantly different