Fig. 5

Snail mediates the GDF-8-induced upregulation of MMP2 expression. A HTR-8/SVneo were transfected with 50 nM control siRNA (si-Ctrl), Snail siRNA (si-Snail), or Slug siRNA (si-Slug) for 48 h, and then treated with 30 ng/mL GDF-8 (G8) for 6 h. The mRNA levels of MMP2, Snail, and Slug were examined by RT-qPCR. B HTR-8/SVneo were transfected with 50 nM control siRNA (si-Ctrl), Snail siRNA (si-Snail), or Slug siRNA (si-Slug) for 48 h, and then treated with 30 ng/mL GDF-8 (G8) for 12 h. The protein levels of MMP2, Snail, and Slug were examined by western blot. C HTR-8/SVneo cells were transfected with 1 µg control vector (pCMV) or vector containing Snail (pCMV-Snail) for 48 and 72 h. The protein levels of MMP2 and Snail were examined by western blot. The results are expressed as the mean ± SEM of at least three independent experiments. Multiple comparisons were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. Values that are statistically different from one another (p < 0.05) are indicated by different letters. The values with any common letter are not significantly different