Fig. 6

25KbPEI stimulates translation of immune related factors in NK cells. A Comparison of -fold changes at the transcriptional (RNA-seq) and translational (Proteomics) levels. B Volcano plot of the entire transcriptome (left) or proteome (right) differentially expressed between C_NK and Chem_NK cells. Factors significantly downregulated are shown in blue, and those that are significantly upregulated are shown in red. The vertical lines denote a -fold change of ± 1.25, and the horizontal lines denotes a P value of 0.05. The x-axis represents the log₂ of the fold change, and the y-axis represents the − log of the P value. C Pie graph showing expression of protein products of significantly regulated genes. The dot plots show upregulated Gene ontology (GO) terms enriched in DEGs, and the number of genes enriched within each GO term is represented by dot size and color. Translation-associated GO terms are shown with a purple border. The x-axis represents the log₂ of the enrichment ratio, and the y-axis represents the − log of the FDR. D Heat map of gene expressions associated with immunity GO terms in protein level (upper panel), and in mRNA level (lower panel). The dotted line represents a -fold change of 1. *P < 0.05; **P < 0.01; ***P < 0.001, and ****P < 0.0001