Fig. 5

Inhibition of eIF4E-mediated translation impairs the effect of 25KbPEI on NK cells. A Expression of the indicated receptors by C_NK and Chem_NK cells was analyzed by flow cytometry. Chem_NK was generated by treating NK-92MI cells with 4EGI-1 (50 μM) for 3 h prior to 25KbPEI treatment. Statistical analysis was conducted using one-way ANOVA with Tukey’s multiple comparisons test. B Migration of C_NK and Chem_NK cells toward ovarian cancer cells in the presence or absence of 4EGI-1 (50 μM) was analyzed in Transwell assay. Statistical analysis was conducted using two-way ANOVA with Sidak’s multiple comparisons test. All experiments were conducted at least three times. *P < 0.05; **P < 0.01; ***P < 0.001, and ****P < 0.0001