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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: mTOR-dependent TFEB activation and TFEB overexpression enhance autophagy-lysosome pathway and ameliorate Alzheimer's disease-like pathology in diabetic encephalopathy

Fig. 6

TFEB overexpression promoted clearance of AD-related proteins in HG-cultured HT22 cells and the effect of TFEB overexpression was inhibited by 3-MA. APP, Aβ42, p-Tau and Tau were measured via western blotting in HT22 cells (A), GAPDH was used as the loading control, n = 3. Representative double staining image of LAMP1 with Aβ42 in HT22 cells with different treatments (B); summary data show LAMP1 and Aβ42 florescent intensities normalized to NG + NULL group (C, D); the co-localization is represented by yellow signals and quantified as Pearson’s correlation coefficient (E). Representative double staining image of LAMP1 with p-Tau in HT22 cells with different treatments (F); summary data show LAMP1 and p-Tau florescent intensities normalized to NG + NULL group (G, H); the co-localization is represented by yellow signals and quantified as Pearson’s correlation coefficient (I). scale bar: 10 μm, n = 15 cells from 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X