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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: mTOR-dependent TFEB activation and TFEB overexpression enhance autophagy-lysosome pathway and ameliorate Alzheimer's disease-like pathology in diabetic encephalopathy

Fig. 5

TFEB overexpression enhanced ALP function of HG-cultured HT22 cells and the effect of TFEB was inhibited by 3-MA. TFEB, LC3, p62, CTSB and LAMP1 and were measured via western blotting in HT22 cells (A), GAPDH was used as the loading control, n = 3. Results of CTSB activity of the HT22 cells, the activity of CTSB was expressed as fluorescent units/ug protein (B), n = 3. Representative confocal images of LC3 puncta in HT22 cells expressing mRFP-GFP-LC3, autophagosomes were labelled by yellow puncta dots due to double staining of green and red fluorescence, autolysosomes were labelled by red puncta only (C), scale bar: 10 μm; quantification of relative LC3 puncta was plotted (D, E) and was normalized to NG + NULL group. n = 9 cells from 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X