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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: mTOR-dependent TFEB activation and TFEB overexpression enhance autophagy-lysosome pathway and ameliorate Alzheimer's disease-like pathology in diabetic encephalopathy

Fig. 3

TFEB nuclear translocation was decreased in T2DM mice and HG-cultured HT22 cells, which was related to decreased TFEB activity and expression. Nucleus (A) and cytosol TFEB (B) were measured via subcellular fractionation experiments by western blotting in the hippocampal of mice, n = 6; Nucleus and cytosol TFEB (C) were measured via subcellular fractionation experiments by western blotting in HT22 cells, n = 3; GAPDH was used as the loading control of cytosol TFEB, Histone-H3 was used as the loading control of nucleus TFEB. HT22 cells were treated with or without high glucose. After immunostaining with TFEB (green) and DAPI (blue), cells were examined by fluorescence microscopy, scale bar: 10 μm. The colocalization of TFEB and DAPI was calculated and analyzed, n = 3. TFEB, p-TFEB, mTOR and p-mTOR were measured via western blotting in the hippocampal of mice (E), n = 6, and HT22 cells (F), n = 3, GAPDH was used as the loading control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X