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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression

Fig. 3

TG2 mediates intracellular 5-HT serotonylates and activates RhoA to promote YAP in colon cancer cells. A After siRNA transfection downregulated TG2 expression in SW480 and SW1116 cells, a western blot was used to analyse YAP and Cyr61 expression in cells treated before with 10 μM 5-HT. B After downregulating RhoA expression in SW480 and SW1116 cells, YAP and Cyr61 expressions in cells were treated with 10 μM 5-HT. For this procedure, a western blot test modelled these expressions. C GST pull-down analysis was used to detect the levels of total and active RhoA in cells stimulated with 5-HT (10 μM) or pre-treated with 100 μM citalopram before 5-HT stimulation. D Immunoprecipitation was performed to analyse the expression of RhoA, 5-HT in cells stimulated with 5-HT (10 μM) or pre-treated with 100 μM citalopram before 5-HT stimulation

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Cell Communication and Signaling

ISSN: 1478-811X