Fig. 2

SERT transports extracellular 5HT entry into colon cancer cells. A CCK-8 assay treated the cells with the broad spectrum 5-HTR inhibitor asenapine (100 μM) and SERT antagonists citalopram (100 μM) or fluvoxamine (100 μM) for 2 h before 5-HT stimulation. In succession to this procedure, cell proliferation was detected by the CCK-8 assay. *, P < 0.05; **, P < 0.01. B Moreover, a western blot analysis of YAP and Cyr61 expression in SW480 and SW1116 cells were treated with 100 μM asenapine or SSRIs citalopram, and fluvoxamine for 2 h before using 5-HT stimulation. C A western blot test was used to stimulate YAP and Cyr61 after knocking down SERT expression in SW480 and SW1116 cells