Fig. 5

Silencing ADAM17 reduces cardiomyocyte apoptosis and remodeling. H9C2 cardiomyocytes were transfected with ADAM17 siRNA or siRNA control (NC) for 6 h and treated with 600 μM hydrogen peroxide for 12 h. A The ADAM17 mRNA expression were detected by RT-qPCR in H9C2 after treatment as above (n = 3). B Western blot analysis of ADAM17, collagenI, collagenIII and TGF-β1 protein level in H9C2 cardiomyocytes (n = 3). C The cell viability was detected by CCK8 assay (n = 4). D Flow cytometric analysis of annexin V/PI double stained cells after hydrogen peroxide treatment (left). Histogram indicating the percentage of later apoptosis cells in 4 groups (right) (n = 3). E, F Fluorescence microscopic images of cells stained with a calcein-AM/PI double staining kit (E). The live (green) and dead (red) cell percentages quantified from fluorescent images (F) (n = 3). Data shown as mean ± SEM. Derived by two-sample t-test, *P < 0.05; **P < 0.01, ***P < 0.001