Fig. 4

Expression pattern of ADAM17 in hydrogen peroxide-treated cardiomyocytes. A CCK8 detects H9C2 cardiomyocyte viability after stimulation with different concentrations. B Western blot measurement of ADAM17 and myocardial remodeling-related makers collagenI, collagenIII and TGF-β1 in H9C2 cardiomyocytes treated with different concentrations of hydrogen peroxide. C Cell viability of H9C2 cardiomyocytes treated with 600 μM hydrogen peroxide for the indicated time was detected by CCK8. D CollagenI, collagenIII and TGF-β1 protein levels of hydrogen peroxide-treated H9C2 cardiomyocytes at the indicated time were detected by western blotting. E RT-qPCR analysis of collagenI, collagenIII and TGF-β1 mRNA levels in hydrogen peroxide-treated H9C2 cardiomyocytes. F Representative images were taken from the above cells stained by Annexin V/propidium iodide(right). Cell apoptosis (%) was measured by cytometry(left). G western blot measurement of ADAM17 level in H9C2 cardiomyocytes treated with different concentrations of hydrogen peroxide for 12 h. H RT-qPCR analysis of ADAM17 mRNA level in H9C2 cardiomyocytes treated with different concentrations of hydrogen peroxide for 12 h. I western blot measurement of ADAM17 level in H9C2 cardiomyocytes treated with 600 μM hydrogen peroxide for the indicated time. J RT-qPCR analysis of ADAM17 mRNA level in H9C2 cardiomyocytes treated with 600 μM hydrogen peroxide for the indicated time. N = 3 independent cell preparations. Data shown as mean ± SEM. Derived by two-sample t-test, *P < 0.05; **P < 0.01, ***P < 0.001