Fig. 2

ADAM17 expression was increased in myocardium of post-MI HF mice. A WT C57BL/6 mice were subjected to MI surgery and sacrificed after 30 days. B–F Echocardiographic analysis images of left ventricular (LV) M-mode echocardiograms 30 days after surgery. LV Internal diastolic diameter (LVIDd) (C), LV internal dimension systole (LVIDs) (D), Ejection fraction (EF) (E), and fractional shortening (FS) (F) were measured from different groups. G Representative Masson’s trichrome staining of the hearts from different groups. H–I WGA staining to measure cardiomyocyte surface area and its quantification. J mRNA expression levels of ADAM17, ANP, BNP, and MyHC of different groups mice heart was measured by RT-qPCR. K Immunofluorescence for cTNT (red) ADAM17 (green) and DAPI (blue) nuclei staining of histological sections in remote and infarct zone of the MI mice heart. CON, WT control; sham, sham operation; MI = MI surgery. N = 6 biological replicates. Data shown as mean ± SEM. Derived by two-sample t-test, *P < 0.05; **P < 0.01, ***P < 0.001