Fig. 6

OMV-induced Mx1 expression is lost after LPS inhibition. THP-1 cells were incubated for 20 h with OMVs (1 µg/mL; Kp or Clear coli (Cc)) alone or in combination with 20 µg/mL Polymyxin B (PB) or left untreated for control. A Mx1 protein expression was determined by Western Blot. A representative result of three biological independent experiments is shown. Mx1 B and CXCL8 C expression was determined by qPCR. Bars show mean values of four independent experiments + SEM. D THP-1 cells were incubated for 20 h with OMVs (1 µg/mL; Kp or Cc) alone or in combination with 20 µg/mL PB or left untreated for control and then additionally infected with A/WSN/33(H1N1) (MOI 0.001). Virus replication was determined by plaque assay 24 h after infection. Bars show mean values of four independent experiments + SEM. Statistics: 1-way ANOVA B–D; *p < 0.05, **p < 0.01, ****p < 0.0001; *compared to control, #compared to KpOMV; ns = not significant; n = 3–4