Fig. 6
From: Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells
The GSDME-N fragment promotes EGFR degradation. A Western blot analysis of FLAG and EGFR levels in H1299 cells. H1299 cells were transfected with pcDNA3.1, pcDNA3.1-FLAG-GSDME (D270A)-HA, pcDNA3.1-FLAG-GSDME-N and treated with or without 2 μM doxorubicin for 24 h. B Western blot analysis of EGFR and GSDME levels in H1299 cells treated with 10 μg/mL cycloheximide (CHX) for 0 h, 6 h, 12 h, and 24 h after transfection with pcDNA3.1 or pcDNA3.1-FLAG-GSDME-N for 24 h. C, D Colony formation efficiency of A549 and H1299 cells after transfection with pcDNA3.1 or pcDNA 3.1-GSDME-N plasmids for 14 days. Data are presented as the mean ± SD and are representative of three independent experiments. The significant differences between the two groups were analyzed by two-sided unpaired Student’s t tests (**p < 0.01, ***p < 0.001)