Fig. 4
From: Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells
GSDME knockdown decreases the protein levels of EGFR and suppresses the phosphorylation of ERK1/2 in NSCLC cells. A, B Western blot analysis of EGFR, phosphorylated ERK1/2, ERK1/2 and GSDME levels in Calu-1, H157 and A549 cells treated with 10 ng/mL EGF for 30 min after transfection with siScr or siGSDME for 48 h. C Western blot analysis of EGFR and GSDME levels in A549 cells transfected with pcDNA3.1, pcDNA3.1-FLAG-GSDME-HA, or pcDNA3.1-FLAG-GSDME (D270A)-HA treated with or without 1 μM doxorubicin for 24 h. D Western blot analysis of EGFR and GSDME levels in H1299 cells transfected with pcDNA3.1 and pcDNA3.1-FLAG-EGFR treated with or without 4 μM doxorubicin for 24 h. E Western blot analysis of EGFR and GSDME in EGFR-depleted A549 cells. GAPDH or β-Actin was used as the loading control