Fig. 2
From: Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells
GSDME knockdown decreases the protein levels of CCND1 and phosphorylation of ERK1/2 in NSCLC cells. A, B Cell cycle distribution of A549 and H1299 cells after transfected siScr or siGSDME for 48 h. The cell cycle distribution was measured by flow cytometry. C Western blot analysis of CCND1 and GSDME levels in Calu-1 cells. D Western blot analysis of CCND1 and GSDME levels in H157 cells. GAPDH was used as the loading control. E Western blot analysis of CCND1 and GSDME levels in A549 cells treated with 10 ng/mL EGF for 0, 10, 20 and 40 min after transfection with siScr or siGSDME for 48 h. β-Actin was used as the loading control. F Western blot analysis of CCND1 and GSDME in H157 cells treated with 10 ng/mL EGF for 30 min after transfection with siScr or siGSDME for 48 h. β-Actin was used as the loading control G Western blot analysis of the phosphorylation of ERK1/2 in A549 and H157 cells treated with 10 ng/mL EGF for 30 min after transfection with siScr or siGSDME for 48 h. ERK1/2 were used as the loading control. Data are presented as the mean ± SD and are representative of three independent experiments. The significant differences between the two groups were analyzed by two-sided unpaired Student’s t tests (**** p < 0.0001)