Fig. 1
From: Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells
GSDME depletion suppresses the proliferation of NSCLC cells in vitro. A GSDME mRNA expression in TCGA cancer samples and normal samples from UALCAN. High expression of GSDME across TCGA cancers: CHOL, GBM, HNSC, LUAD, LUSC and PCPG (Red color). Low expression of GSDME across TCGA cancers: BLCA, BRCA, CESE, KICH, PAAD, PRAD and UCEC (Blue color). B Relative mRNA level of GSDME in TCGA LUAD samples (NT, n = 58; TP, n = 533; p < 0.05) and LUSC samples (NT, n = 49; TP, n = 502; p < 0.0001) compared with normal samples. C The protein levels of GSDME in A549, Calu-1, H1299, H1792 and H157 cells were measured by Western blot analysis. β-Actin was used as the loading control. D EdU incorporation measured by flow cytometry in Calu-1 cells transfected with siScr or siGSDME for 48 h. E Colony formation efficiency of Calu-1 cells after transfection with scrambled siRNA (siScr) or siRNA targeting GSDME (siGSDME) for 14 days. F The RNAi efficiency of Calu-1 cells was measured by Western blot analysis. β-Actin was used as the loading control. Data are presented as the mean ± SD and are representative of three independent experiments. The significant differences between the two groups were analyzed by two-sided unpaired Student’s t tests (*p < 0.05, ***p < 0.001, ****p < 0.0001)