Fig. 4

ER stress induction and both repression of HCV replication and reversion of ER stress/PKA/GSK-3β/β-catenin pathway by ER stress inhibitor TUDCA in both chronic HCV-infected Huh7.5 cells and R4-GFP HCV replicon Huh7 cells. A Whole cell lysates were taken from chronic HCV-infected Huh7.5 cells (d40 and d72) and uninfected control cells for western blotting with indicated antibodies. B Chronic HCV-infected Huh7 cells (d163) were treated with TUDCA. After 48 h, cell lysates were collected for western blotting with indicated antibodies. C Whole cell lysates were taken from R4-GFP HCV replicon Huh7 cells and parental cells (without HCV replicon) for western blotting with indicated antibodies. D R4-GFP HCV replicon Huh7 cells were treated with TUDCA. After 96 h, cell lysates were collected for western blotting with indicated antibodies