Fig. 3

Both repression of HCV replicon replication and reversion of PKA/GSK-3β/β-catenin pathway by PKA inhibition with H89 in R4-GFP HCV replicon Huh7 cells. A Cytotoxicity of H89 in R4-GFP HCV replicon Huh7 cells in a concentration-dependent manner by MTT assay. B R4-GFP HCV replicon Huh7 cells were treated with low dose of H89 (8 uM). After 14 days and 25 days, cell lysates were collected for western blotting with indicated antibodies. UT, untreated. C R4-GFP HCV replicon Huh7 cells were treated with low dose of H89 (8 μM). After 14 days and 25 days, fluorescence microscopy was taken. Scale bar = 50 μm. UT, untreated