Fig. 2

Both repression of HCV replication and reversion of PKA/GSK-3β/β-catenin pathway by PKA inhibition with H89 in chronic HCV-infected Huh7.5 cells. A Cytotoxicity of H89 after 48 h of treatment in Chronic HCV-infected Huh7.5 cells (d129) was performed by MTT assay. B Chronic HCV-infected Huh7.5 cells (d129) were treated with high doses of H89 (20 and 40 μM). After 48 h, cell lysates were collected for western blotting with indicated antibodies. It is noticed that the internal loading control for western blot analysis are in Fig. 1D. C Chronic HCV-infected Huh7.5 cells (d129) were treated with high doses of H89 (20 and 40 μM). After 48 h, fluorescence microscopy was taken. Scale bar = 25 μm. D Chronic HCV-infected Huh7.5 cells (d168) were treated with low doses of H89 (6 and 8 μM). After 12 days, cell lysates were collected for western blotting with indicated antibodies. E Cell counting assay was performed in chronic HCV-infected Huh7.5 cells treated with low doses of H89 for 12 days