Table 2 Identified bull sperm proteins showing S-nitrosylation (S-nitro), denitrosylation (Denitro), S-glutathionylation (S-gluta), deglutathionylation (De-gluta), tyrosine phosphorylation (pTyr) and dephosphorylation (De-pTyr)

TCA cycle

Aconitase 2, mitochondrial precursor

ACO2

1*

2*

3*

2-Oxoglutarate dehydrogenase, mitochondrial precursor

OGDH

4

Dihydrolipoyl dehydrogenase, mitochondrial

DLD

10*

11*

245*

249*

Fumarate hydratase, mitochondrial

FH

22*

23*

24*

111*

112*

113*

Citrate synthase, mitochondrial precursor

CS

27*

28*

376*

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor

IDH3A

118*

L-lactate dehydrogenase C chain isoform X2

LDHC

116*

Energy metabolism

ATP synthase subunit alpha, mitochondrial isoform X1

ATP5F1A

5*

6*

8*

E Chain E, ATP synthase D chain, mitochondrial

ATP5ME

123*

F chain F, ATP synthase subunit beta, mitochondrial

ATP5F1B

127

C chain C, bovine mitochondrial F1-ATPase

1COW

261*

A chain A, cytochrome B-c1 complex subunit 1, mitochondrial

UQCRC1

16*

255*

N chain N, cytochrome b-c1 complex subunit 1, mitochondrial

UQCRC1

21*

B chain B, cytochrome B-c1 complex subunit 2, mitochondrial

UQCRC2

19*

104

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial isoform X3

NDUFS8

39*

C chain C, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

NDUFS3

143*

Pyruvate kinase PKM isoform X1

PKM

7*

Alpha enolase

ENO1

114*

240*

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit isoform X1

PPP1CC

35*

37*

242*

Organelle component assembly (general)

MICOS complex subunit MIC60 isoform X4

IMMT

14*

Prohibitin isoform X1

PHB

126

Ras-related protein Rab-2A isoform X3

RAB2A

122*

Ras-related protein Rab-2A

RAB2A

51*

Transitional endoplasmic reticulum ATPase

VCP

130

Organelle component assembly (actin polymeryzation)

Actin-like protein 7A

ACTL7A

13*

F-actin-capping protein subunit beta isoform X3

CAPZB

31*

A chain A, actin, cytoplasmic 1

ACTB

142

Fascin-3 isoform X2

FSCN3

234*

Fertilization

Disintegrin and metalloproteinase domain-containing protein

ADAM2

17

Zonadhesin

ZAN

25*

26*

Zona pellucida-binding protein 1 isoform X1 (sperm inner acrosomal membrane protein IAM38)

ZPBP1

106*

Sperm adhesion molecule 1 isoform X2

SPAM1

100*

101*

Izumo sperm-egg fusion protein 4

IZUMO4

43

44

49*

47*

48*

120*

121*

Acrosin-binding protein isoform X1

ACRBP

38*

46*

Sperm equatorial segment protein 1 precursor

SPESP1

250*

Postacrosomal sheath WW domain-binding protein (PAWP)

WBP2NL

32*

Redox process

Glycerol-3-phosphate dehydrogenase, mitochondrial isoform X1

GPD2

12*

107*

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform X1

GAPDHS

18*

20*

102

103

141

Glutathione S-transferase omega-2 isoform X1

GSTO2

41*

Superoxide dismutase [Mn], mitochondrial precursor

SOD2

42*

45*

50*

L-amino-acid oxidase isoform X2

IL4I1

346

Sperm flagellum

Tektin 3

TEKT3

9*

Tektin-2 isoform X1

TEKT2

246*

Radial spoke head 14 homolog isoform X1

RSPH14

33*

Dynein, axonemal, light intermediate chain 1-like

DNALI1

108*

Tubulin alpha-3 chain isoform X3

TUBA3A

132*

Outer dense fiber protein 2 isoform X20

ODF2

124*

305*

329*

331*

Outer dense fiber protein 2 isoform X17

ODF2

325*

Nucleoside diphosphate kinase homolog 5 isoform X1

NME5

52*

Phosphatidylethanolamine-binding protein

PEBP1

110*

Sperm flagellum (cAMP/PKA pathway)

H chain H, cAMP-dependent protein kinase type I-alpha regulatory subunit (PKA)

PRKAR1A

347*

348*

349*

A-kinase anchor protein 4

AKAP4

317

343

A-kinase anchor protein 3

AKAP3

344

Chaperonin

T-complex protein 1 subunit alpha isoform X1

TCP1

15*

DnaJ homolog subfamily B member 13

DNAJB13

30*

60 kDa heat shock protein, mitochondrial

HSPD1

133*

366

Heat shock-related 70 kDa protein 2

HSPA2

135*

TPA: T-complex protein 1 subunit zeta-2

CCT6B

277

Other

Casein kinase II subunit alpha

CSNK2A1

105

B chain B, seryl-trna synthetase

SARS2

238*

Fructose-1,6-bisphosphatase, partial

FBP1

117*

Leucine rich repeat containing 37A-like protein

LRRC37A

345*

Ubiquitin carboxyl-terminal hydrolase isozyme L1 isoform X1

UCHL1

40*

Cytosolic 5′-nucleotidase 1B isoform X2

NT5C1B

237*

Cytosolic 5′-nucleotidase 1B isoform X1

NT5C1B

235*

Sperm-associated antigen 6

SPAG6

239*