Table 2 Methods for studying tRFs

tRFdb

Can display coordinates and names of retrieved tRF sequences from various sources

http://genome.bioch.virginia.edu/trfdb

tsRFun

Construct interaction networks among tsRNAs, miRNAs, and mRNAs, offering diverse real-time online tools for tsRNA research

https://rna.sysu.edu.cn/tsRFun/

https://biomed.nscc-gz.cn/DB/tsRFun/

DBtRend

Provides mature tRNA expression profiles across various biological conditions and enables users to identify biological conditions or tissue types associated with a specific differentially expressed tRNA

https://trend.pmrc.re.kr

tRFexplorer

Allows users to investigate the potential biological roles of tRNA-derived ncRNAs, without any direct experimental evidence

https://trfexplorer.cloud/

tRic

Includes codon and amino acid frequency data for all human protein-coding genes

https://hanlab.uth.edu/tRic/

http://bioinfo.life.hust.edu.cn/tRic/

MINTbase v2.0

Contains nuclear and mitochondrial tRFs from multiple human tissues

http://cm.jefferson.edu/MINTbase/

tRF2Cancer

can accurately recognize tRFs and evaluate their expression in diverse cancers

http://rna.sysu.edu.cn/tRFfinder/

OncotRF

can be used to identify diagnostic and prognostic biomarkers for cancers

http://bioinformatics.zju.edu.cn/OncotRF

tsRBase

can help to describe specific tsRNA expression patterns under different backgrounds

http://www.tsrbase.org