Fig. 3

Altered transcriptome in IQM-induced psoriasis mouse models. Kit^wsh/wsh mice (n = 3) and their littermate controls (n = 3) were treated with IMQ and Vaseline topically for four consecutive days. Skin samples were collected on Day 5 for transcriptome analysis. a Volcano plot of differentially expressed genes (DEGs) in each group (|log₂FC|≥ 1.5 and p ≤ 0.05; red: up-regulated DEGs; green: down-regulated DEGs). b Hierarchical Clustering analysis of DEGs (red: high relative expression; blue: low relative expression). Expression levels were subjected to Z score scaling within each sample for visualisation purposes. c Gene expression levels of KCNE3, LTF, MYOG, NMRK2, MS4A2, PROK2, SLURP1, GJA5, FPR2, and TPH1 were measured using real-time PCR. Data were normalised to GADPH expression levels and reported in relative expression units. Data are represented by mean ± SEM from three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001. d Venn diagram of the overlapping DEGs from the four experimental groups (WT/Vas vs WT/IMQ; WT/ Vas vs Wsh/ Vas; Wsh/ Vas vs Wsh/IMQ; WT/IMQ vs Wsh/IMQ). e–g GO enrichment analysis of DEGs in biological process (e), cellular components (f), and molecular function (g). h KEGG pathway enrichment analysis of the DEGs