Fig. 7

Effect of MLK3 inhibition on activities of IKK and GSK3α: N2a cells were differentiated in the absence (MF; insulin sensitive) or chronic presence of 100 nM insulin (MFI; insulin resistant) for 3 days and treated with or without 10 µM URMC099 for 2 h followed by 100 nM insulin for 30 min. Treated cells were lysed and subjected to western immunoblotting followed by probing with relevant primary antibodies. Bar represents relative change in A pIKKα/β (Ser179/180), B pGSK3α (Ser21). Experiments were executed three times and a representative western blot is shown. Data expressed are mean ± SE. ***p < 0.001 compared to Lane 2, ^θθθp < 0.001 compared to lane 4. A IKKα, B GSK3α was used as a loading control. Open bars: MF, filled bars: MFI, IB Immunoblot