Fig. 2

Effect of insulin stimulation on expression of PP1α and PP1γ in insulin sensitive and insulin resistant condition: Differentiated A and B N2a cells C and D SH-SY5Y cells were treated with or without 100 nM insulin for 30 min and lysed E and F ND and HFD mice brains were lysed G and H N2a cells were differentiated in insulin sensitive (MF) or in insulin resistant (MFI) conditions for 3 days and treated with or without 100 nM insulin for 30 min and were lysed, I and J SH-SY5Y cells were differentiated in insulin sensitive (MF) or in insulin resistant (MFI) conditions for 4 days and were treated with or without 100 nM insulin for 30 min and lysed. Resulting lysates were subjected to western immunoblotting and probed with relevant antibodies Bar represents relative change in expression of PP1α (A, C, E, G and I) and PP1γ (B, D, F, H and J). Experiments were executed three times and representative western blot is shown. Data expressed are mean ± SE. Open bars: MF, filled bars: MFI, IB Immunoblot, ND Normal diet, HFD High-fat-fed-diet. α-Tubulin was used as loading control