Fig. 6

Inhibition of p38 decreases the expressions of mitochondrial fusion proteins induced by FGF19 in chondrocytes. a Representative western blotting showing the expression change of Opa1, Mfn1 and Mfn2 in chondrocytes induced by SB203580 (10 µM) in the presence of FGF19 (200 ng/ml) and KLB (200 ng/ml). The images were chosen based on three independent experiments (n = 3). b Quantification of Opa1, Mfn1 and Mfn2 by western blotting in (a). c Representative immunofluorescent staining showing the change in the expression and distribution of Opa1 in chondrocytes induced by SB203580 (10 µM) in the presence of FGF19 (200 ng/ml) and KLB (200 ng/ml). The images were chosen based on three independent experiments (n = 3). Red, Opa1; Green, F-actin; Blue, nucleus. d Representative immunofluorescent staining showing the change in the distribution of Mfn2 in chondrocytes induced by SB203580 (10 µM) in the presence of FGF19 (200 ng/ml) and KLB (200 ng/ml). The images were chosen based on three independent experiments (n = 3). Red, Mfn2; Green, F-actin; Blue, nucleus. e Quantification of fluorescence intensity of Opa1 and Mfn2 in chondrocytes induced by SB203580 (10 µM) in the presence of FGF19 (200 ng/ml) and KLB (200 ng/ml). The data were based on at least eight cells from three independent experiments. f Representative immunofluorescent staining showing the changes of morphology mitochondrial network in living chondrocytes induced by SB203580 (10 µM) in the presence of FGF19 (200 ng/ml) and KLB (200 ng/ml) for 72 h. Image J shows the change of mitochondrial network morphology analysis in cyan boxes. The images were chosen based on three independent experiments (n = 3). Red, mitochondrial network; Blue, nucleus. g Quantification of mitochondrial number (per cell) and mitochondrial elongated number (per cell) in chondrocytes induced by SB203580 (10 µM) in the presence of FGF19 (200 ng/ml) by Image J. Quantitative analyses were based on three independent experiments (n = 3). The data in e and g were shown as box (from 25, 50 to 75%) and whisker (minimum to maximum values) plots. The significant difference analysis in b, e and g were based on Student T-test