Fig. 6

Inhibition of p38MAPK phosphorylation and its effect on CRC cell migration and MMP-2/MMP-9 level/release after PMP uptake. A Representative Western blots of phospho-p38MAPK in CRC cells after 10 min of incubation with PMPs in the absence or presence of the inhibitor SB202190. B Migration of CRC cells through gelatine-coated Boyden chambers after 4 h of incubation with the inhibitor SB202190. Quantified data (presented and statistically analysed as in Fig. 1) of the percentage of gelatine-migrated cells compared to the control (nontreated cells, 100%, marked with dashed horizontal bars). *P < 0.05, N = 5. C Representative Western blots of MMP-2 and MMP-9 in CRC cells after 4 h of incubation with the inhibitor SB202190. D Amount of MMP-9 released from CRC into conditioned medium after 4 h of incubation with PMPs in the absence or presence of the inhibitor SB202190, as measured by ELISA. The data are presented and statistically analysed as in Fig. 1. **P < 0.01, *P < 0.05. N = 6