Fig. 3

Effect of PMP internalization on the migration and invasion potential of HT29, SW480 and SW620 cells. A Wound closure (recovery) of CRC cells, control and preincubated for 4 h with PMPs after a 24-h migration period. *P < 0.05, N = 5. B and C migration and invasion, respectively, of HT29, SW480 and SW620 cells through uncoated (B—migration) or Matrigel-coated (C—invasion) after 4 h of incubation with PMPs in the presence or absence of SDF-1 and/or the CXCR4 antagonist AMD3100. For B and C, nontreated cells were considered 100% (dashed horizontal bar). The asterisk denotes the significance of the inhibition of migration and invasion of cells treated with AMD3100. Statistics were calculated with one-way ANOVA followed by Tukey’s test for multiple comparisons: *P < 0.05, N = 5