Fig. 7

HIF1α directly regulates KCNJ2 transcription by binding to its promoter. A Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the mRNA levels of KCNJ2 in U2OS and 143B cells under hypoxic conditions. B, C Western blotting was used to detect the protein levels of KCNJ2 in U2OS and 143B cells under hypoxic conditions. D Motif of HIF1α. E Hypothetical binding site of HIF1α in the KCNJ2 promoter (F) CHIP-qPCR was used to detect the binding site of HIF1α in the KCNJ2 promoter. G, H Double luciferase reporter assays detected fluorescence intensity in NC and si-HIF1α cells transfected with wildtype/mutated plasmids under normoxia and hypoxia. I qRT-PCR was used to detect the mRNA levels of KCNJ2 in NC and si-HIF1α cells under normoxic and hypoxic conditions. J, K western blotting was used to detect the protein levels of KCNJ2 in NC and si-HIF1α cells under normoxia and hypoxia. *, P < 0.05; **, P < 0.01