Fig. 6
From: KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma
Knockdown of HIF1α reduces the oncogenic effects of KCNJ2-overexpression on OS cell. OS cells were treated as follows: negative control siRNAs (NC) + empty vector; NC + KCNJ-overexpression; targeting HIF1α siRNAs (si-HIF1α) + vector; si-HIF1α + KCNJ2-overexpression. A–C Protein levels of HIF1α and KCNJ2 were detected by western blotting in each group cells. D, E Wound healing assays were used to detect the migration rate of each group. F, G Transwell assays were used to detect the invasion rate of each group. **, P < 0.01