Fig. 5
From: KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma
KCNJ2 reduced the ubiquitination degradation of HIF1α. A–B Expression of HIF1α and CA9 was detected by western blotting after KCNJ2-inhibition. C–D Expression of HIF1α and CA9 was detected by western blotting after KCNJ2-overexpression. E–F The degradation rate of HIF1α in 143B cells with empty vector and KCNJ2-overexpression. G–H Western blotting indicated that KCNJ2-inhibition markedly reduced HIF1α and CA9 expression in 143B cells, while the 26S protease inhibitor MG132 relieved this downregulation of HIF1α and CA9 that was induced by KCNJ2-inhibition. I Ubiquitination levels of HIF1α in 143B cells with empty vector and KCNJ2-overexpression. **, P < 0.01