Fig. 4
From: KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma
KCNJ2 binds to KCNJ2 and is co-expressed with KCNJ2 in OS tissues. A Coomassie brilliant blue staining exhibited the landscape of KCNJ2-interacting proteins. Input line was added the total proteins in 143B cells, IgG line was added the proteins enriched by IgG antibodies in 143B cells, while output line was added the proteins enriched by KCNJ2 antibodies in 143B cells. B Mass spectrometry identified HIF1α as KCNJ2 interacting protein of KCNJ2. For the figures of secondary mass spectrum, red lines mean water peak, while blue lines mean NH3 peak. C Immunoprecipitation demonstrated the interaction between KCNJ2 and HIF1α. D KCNJ2 mRNA levels were co-expressed with HIF1A mRNA levels. E KCNJ2 protein levels were co-expressed with HIF1α protein levels