Fig. 6
From: PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis
PcTrim decreases dynamin expression by inhibiting the activity of AP1. A–D The expression of dynamin in crayfish injected with anti-Trim antibody (A), His-Trim (B), anti-AP1 antibody (C) or dsAP1 (D) was analyzed via qRT‒PCR. Anti-Actin antibody, His-Tag and dsGFP were used as controls. E The dynamin RNAi efficiency was analyzed via qRT‒PCR. F Hemocyte phagocytosis was observed under a fluorescence microscope after dynamin RNAi treatment. G The phagocytosis rate of VP28 after dynamin RNAi treatment. (H) The 5’ untranslated region of dynamin was analyzed using the online Promoter Scan tool. Two AP1 binding sites were found in the genomic regulatory region of dynamin. I A ChIP assay was used to detect the AP1 binding sites in the dynamin promoter. J–M The expression level of IE1 in crayfish injected with anti-Trim antibody J, His-Trim K, anti-AP1 antibody L or dsAP1 M was analyzed via qRT‒PCR. The asterisk indicates a significant difference, p < 0.05